Trometamol CAS 77-86-1 Tris Tromethamine Tris(hydroxymethyl)aminomethane

Tromethane
THAM
Tris base
Tris buffer
Assay 99.5%min
Packing 25kg/drum

Tris(hydroxymethyl)aminomethane

Name	Trometamol
Synonyms	Tris(hydroxymethyl)aminomethane; Tromethamine; Tromethane; THAM
Molecular Structure
Molecular Formula	C₄H₁₁NO₃
Molecular Weight	121.14
CAS Registry Number	77-86-1 (83147-39-1)
EC Number	201-064-4

Specification:

Item	Specification
Appearance	White crystalline powder
Appearance of Solution (5% aq.)	Colorless and Clear
Identification IR	Conforms to the Reference Spectrum
pH	10.0~11.5
Melting point	168.0ºC ~172.0ºC
Sulfated Ash/Residue on ignition	0.05% Max
Nickel (Ni)	2 ppm Max
Iron(Fe)	1 ppm Max
Heavy metals(as Pb)	5 ppm Max
Chloride	3 ppm Max
Related Substances	Passes Test
Loss on drying	0.2% Max
Abs(260nm,40%water)	0.07 max
Assay(dried basis)	99.5%~100.5%

Uses:

1.Biochemistry and Molecular Biology: Buffer systems used for preparing electrophoresis buffers (such as TAE, TBE),

cell culture media, protein purification, and nucleic acid research.

2.PH regulation and stability: Due to its buffering range (pH 7.0-9.0) close to physiological pH, it is commonly used to

maintain the acid-base stability of biochemical reaction systems.

3.Analytical Chemistry: Used as a standard substance for calibrating pH meters.

4.Other fields: also used in pharmaceuticals, cosmetics, and as a chemical synthesis intermediate.

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